サイクリンE1
機能
CCNE1遺伝子によってコードされるサイクリンE1タンパク質は、高度に保存されたサイクリンファミリーに属する。このファミリーのメンバーは、細胞周期を通じてその存在量に劇的かつ周期的な変化が生じることで特徴づけられる。サイクリンはCDKの調節因子として機能する。さまざまなサイクリンはそれぞれ異なる発現と分解のパターンを示し、有糸分裂の各イベントの時間的な調整に寄与する。サイクリンE1はCDK2の調節サブユニットとして機能し、CDK2の活性は細胞周期のG1期からS期への移行に必要である。サイクリンE1はG1期とS期の境界時点で蓄積し、細胞がS期を進行するにつれて分解される。CCNE1遺伝子の過剰発現は多くの腫瘍でみられ、ゲノム不安定性をもたらすことで腫瘍形成に寄与している可能性がある。サイクリンE1はNPATと結合し、そのリン酸化に関与していることが知られている。NPATは細胞周期によって調節されたヒストン遺伝子発現に関与しており、Rbタンパク質が不在時の細胞周期の進行の促進に重要な役割を果たすタンパク質である[6]。
相互作用
サイクリンE1は次に挙げる因子と相互作用することが示されている。
出典
- 1 2 3 GRCh38: Ensembl release 89: ENSG00000105173 - Ensembl, May 2017
- 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000002068 - Ensembl, May 2017
- ↑ Human PubMed Reference:
- ↑ Mouse PubMed Reference:
- ↑ “Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast”. Cell 66 (6): 1197–206. (October 1991). doi:10.1016/0092-8674(91)90042-W. PMID 1833066.
- ↑ “Entrez Gene: CCNE1 cyclin E1”. 2022年2月7日閲覧。
- 1 2 3 4 5 6 “Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI-SNF complex, and alters the ability of BRG1 to induce growth arrest”. Mol. Cell. Biol. 19 (2): 1460–9. (February 1999). doi:10.1128/mcb.19.2.1460. PMC 116074. PMID 9891079.
- ↑ “Roles of active site residues and the NH2-terminal domain in the catalysis and substrate binding of human Cdc25”. J. Biol. Chem. 271 (9): 5118–24. (March 1996). doi:10.1074/jbc.271.9.5118. PMID 8617791.
- 1 2 “CRM1/Ran-mediated nuclear export of p27(Kip1) involves a nuclear export signal and links p27 export and proteolysis”. Mol. Biol. Cell 14 (1): 201–13. (January 2003). doi:10.1091/mbc.E02-06-0319. PMC 140238. PMID 12529437.
- ↑ “Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells”. Genes Dev. 13 (18): 2375–87. (September 1999). doi:10.1101/gad.13.18.2375. PMC 317026. PMID 10500095.
- 1 2 “Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle”. Science 257 (5077): 1689–94. (September 1992). doi:10.1126/science.1388288. PMID 1388288.
- 1 2 “P21Waf1/Cip1 dysfunction in neuroblastoma: a novel mechanism of attenuating G0-G1 cell cycle arrest”. Cancer Res. 63 (13): 3840–4. (July 2003). PMID 12839982.
- ↑ “mTOR-dependent activation of the transcription factor TIF-IA links rRNA synthesis to nutrient availability”. Genes Dev. 18 (4): 423–34. (February 2004). doi:10.1101/gad.285504. PMC 359396. PMID 15004009.
- ↑ “NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry”. J. Biol. Chem. 275 (33): 25411–7. (August 2000). doi:10.1074/jbc.M001676200. PMID 10827081.
- ↑ “A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins”. Biochem. Biophys. Res. Commun. 266 (1): 115–22. (December 1999). doi:10.1006/bbrc.1999.1777. PMID 10581175.
- ↑ “The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts with cyclins A and E”. Genes Dev. 7 (12A): 2366–77. (December 1993). doi:10.1101/gad.7.12a.2366. PMID 8253383.
- ↑ “BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines”. Cancer Res. 60 (21): 6171–7. (November 2000). PMID 11085541.
関連文献
- “[Prognostic importance of altered expression of cell cycle regulators in lung cancer]”. Nippon Rinsho. 60 Suppl 5: 267–71. (2003). PMID 12101670.
- “A dual role of cyclin E in cell proliferation and apoptosis may provide a target for cancer therapy.”. Current Cancer Drug Targets 4 (1): 65–75. (2004). doi:10.2174/1568009043481669. PMC 1307511. PMID 14965268.
- “Regulation of retinoblastoma protein functions by ectopic expression of human cyclins.”. Cell 70 (6): 993–1006. (1992). doi:10.1016/0092-8674(92)90249-C. PMID 1388095.
- “Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle.”. Science 257 (5077): 1689–94. (1992). doi:10.1126/science.1388288. PMID 1388288.
- “Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family.”. Cell 66 (6): 1217–28. (1991). doi:10.1016/0092-8674(91)90044-Y. PMID 1833068.
- “Chromosomal mapping of the genes for the human cell cycle proteins cyclin C (CCNC), cyclin E (CCNE), p21 (CDKN1) and KAP (CDKN3).”. Cytogenet. Cell Genet. 69 (3–4): 190–2. (1995). doi:10.1159/000133960. PMID 7698009.
- “Human cyclin E, a nuclear protein essential for the G1-to-S phase transition.”. Mol. Cell. Biol. 15 (5): 2612–24. (1995). doi:10.1128/mcb.15.5.2612. PMC 230491. PMID 7739542.
- “Alternative splicing of human cyclin E.”. J. Cell Sci.. 107 ( Pt 2) (2): 581–8. (1994). doi:10.1242/jcs.107.2.581. PMID 8207080.
- “Analysis of p107-associated proteins: p107 associates with a form of E2F that differs from pRB-associated E2F-1.”. J. Virol. 67 (12): 7641–7. (1993). doi:10.1128/JVI.67.12.7641-7647.1993. PMC 238233. PMID 8230483.
- “The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts with cyclins A and E.”. Genes Dev. 7 (12A): 2366–77. (1994). doi:10.1101/gad.7.12a.2366. PMID 8253383.
- “Roles of active site residues and the NH2-terminal domain in the catalysis and substrate binding of human Cdc25.”. J. Biol. Chem. 271 (9): 5118–24. (1996). doi:10.1074/jbc.271.9.5118. PMID 8617791.
- “Regulation of the cyclin E gene by transcription factor E2F1.”. Proc. Natl. Acad. Sci. U.S.A. 92 (26): 12146–50. (1996). doi:10.1073/pnas.92.26.12146. PMC 40313. PMID 8618861.
- “Regulation of cyclin E transcription by E2Fs and retinoblastoma protein.”. Oncogene 12 (6): 1173–80. (1996). PMID 8649818.
- “Cyclin-dependent kinases are inactivated by a combination of p21 and Thr-14/Tyr-15 phosphorylation after UV-induced DNA damage.”. J. Biol. Chem. 271 (22): 13283–91. (1996). doi:10.1074/jbc.271.22.13283. PMID 8662825.
- “Cyclin-binding motifs are essential for the function of p21CIP1.”. Mol. Cell. Biol. 16 (9): 4673–82. (1996). doi:10.1128/MCB.16.9.4673. PMC 231467. PMID 8756624.
- “Molecular cloning and chromosomal localization of the human cyclin C (CCNC) and cyclin E (CCNE) genes: deletion of the CCNC gene in human tumors.”. Genomics 32 (2): 253–9. (1997). doi:10.1006/geno.1996.0112. PMID 8833152.
- “Activation of cyclin E/CDK2 is coupled to site-specific autophosphorylation and ubiquitin-dependent degradation of cyclin E.”. EMBO J. 15 (16): 4182–93. (1996). doi:10.1002/j.1460-2075.1996.tb00793.x. PMC 452142. PMID 8861947.
- “Activation of human B-MYB by cyclins.”. Proc. Natl. Acad. Sci. U.S.A. 94 (2): 532–6. (1997). doi:10.1073/pnas.94.2.532. PMC 19547. PMID 9012818.
- “Cyclin ET, a new splice variant of human cyclin E with a unique expression pattern during cell cycle progression and differentiation.”. Nucleic Acids Res. 25 (11): 2098–105. (1997). doi:10.1093/nar/25.11.2098. PMC 146711. PMID 9153308.